Fig. 5.

Phosphorylation of EphA2 on S897 regulates the subcellular localization of Ephexin4. (A) MCF-7 cells were transfected with EphA2-WT or EphA2-SA. At 24 h after transfection, they were fixed and subjected to immunofluorescent staining with anti-EphA2 and anti-Ephexin4 antibodies. The right panels show the merge of the two images with Ephexin4 (magenta) and EphA2 (green). (B) MCF-7 cells were transfected with GFP together with control luciferase shRNA (shControl) or Ephexin4 shRNA (shEphexin4). At 72 h after transfection, they were fixed and subjected to immunofluorescent staining with anti-Ephexin4 antibody. The right panels show the merge of the two images with Ephexin4 (magenta) and GFP (green). Bars, 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)