Table 3.
Average ΔΔintfor ABF1–ABRE complex for various mutations carried out in silicoat different positions in the ABRE sequence. The mutated position is indicated in bold.
| Type | Sequence | Change in interaction energy (ΔΔGint) kcal/mol (mutant-wildtype) |
|---|---|---|
| Wild type | G G A C A C G T G G C C C | |
| Mutated | ||
| At 5′ end | G G TC A C G T G G C C C | 1.09 ± 0.05 |
| At 5′ end | G G GC A C G T G G C C C | 0.32 ± 0.00 |
| At 5′ end | G G CC A C G T G G C C C | −0.09 ± 0.01 |
| At CACGTG | G G A T A C G T G G C C C | 0.06 ± 0.00 |
| At CACGTG | G G A GA C G T G G C C C | 1.91 ± 0.13 |
| At CACGTG | G G A AA C G T G G C C C | 0.67 ± 0.09 |
| At CACGTG | G G A C GC G T G G C C C | 0.03 ± 0.08 |
| At CACGTG | G G A C TC G T G G C C C | 1.16 ± 0.00 |
| At CACGTG | G G A C CC G T G G C C C | 1.30 ± 0.00 |
| At CACGTG | G G A C A AG T G G C C C | 0.60 ± 0.01 |
| At CACGTG | G G A C A GG T G G C C C | 1.21 ± 0.01 |
| At CACGTG | G G A C A TG T G G C C C | 2.25 ± 0.00 |
| At CACGTG | G G A C A C AT G G C C C | 2.71 ± 0.09 |
| At CACGTG | G G A C A C CT G G C C C | 2.45 ± 0.11 |
| At CACGTG | G G A C A C TT G G C C C | 1.85 ± 0.14 |
| At CACGTG | G G A C A C G CG G C C C | 0.35 ± 0.00 |
| At CACGTG | G G A C A C G GG G C C C | 0.31 ± 0.29 |
| At CACGTG | G G A C A C G AG G C C C | 0.74 ± 0.01 |
| At CACGTG | G G A C A C G T TG C C C | 0.89 ± 1.09 |
| At CACGTG | G G A C A C G T AG C C C | 1.44 ± 0.00 |
| At CACGTG | G G A C A C G T CG C C C | 1.93 ± 0.12 |
| At 3′ end | G G A C A C G T G AC C C | 1.59 ± 0.01 |
| At 3′ end | G G A C A C G T G TC C C | 1.05 ± 0.02 |
| At 3′ end | G G A C A C G T G CC C C | 2.00 ± 0.02 |
| Null mutation | G G A C C T A C A GC C C | 5.99 ± 0.49 |