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. 2013 Jan 11;3:71–77. doi: 10.1016/j.fob.2013.01.001

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative CommonsAttribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Fig. 3 — Adherence of R. sphaeroides NCIB 8253 strains. (A) Viable cells growing in biofilm attached to 68.1 mm² pegs following 5 days aerobic growth at 34 °C. WT, wild type; Fdp1, Fdp2 and Fdp3, mutants possessing an insertionally-inactivated fdp gene; control, uninoculated medium (incorporating extremely low cell numbers that arise upon sonication). Standard deviation derived from nine replicate samples. (B) Biofilm formation during complementation studies of Fdp1 using pRKfdp determined by crystal violet staining, and expressed as a percentage of total culture growth in wells. Culturing was after 5 days at 34 °C. Fdp1/pRKfdp is a complementation by Fdp1 harbouring fdp inserted on pRK415. Standard deviation derived from nine replicate samples. Similar results were obtained for the other mutants.