Table 1. Data for native and SeMet N protein crystals.
Native N protein | SeMet N protein | |
---|---|---|
Beamline | BL-1A, Photon Factory | BL41XU, SPring-8 |
Wavelength (Å) | 1.0000 | 0.9790 |
Detector | Quantum 270 | Rayonix MX225HE |
Crystal-to-detector distance (mm) | 360 | 280 |
Rotation range per image (°) | 1 | 1.4 |
Total rotation range (°) | 180 | 360 |
Exposure time per image (s) | 2 | 1 |
Space group | P21 | P21 |
Unit-cell parameters (Å,°) | a = 66.8, b = 97.2, c = 72.0, β = 112.8 | a = 66.5, b = 96.3, c = 72.1, β = 113.4 |
Mosaicity (°) | 0.97–1.46 | 0.56–1.77 |
Resolution range (Å) | 50.0–3.25 (3.37–3.25) | 50.0–2.70 (2.80–2.70) |
Total No. of reflections | 46995 | 169351 |
No. of unique reflections | 13251 (1330) | 22739 (2268) |
Completeness (%) | 99.4 (99.7) | 99.9 (99.9) |
Multiplicity | 3.5 (3.6) | 7.4 (7.4) |
R merge † (%) | 8.1 (58.9) | 12.9 (68.6) |
〈I〉/〈σ (I)〉 | 14.2 (1.5) | 15.3 (2.2) |
R merge = .