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. 2013 Apr 11;4(4):e583. doi: 10.1038/cddis.2013.98

Figure 3.

Figure 3

Menin and SUV39H1 contribute to GBX2 repression. (a) The cDNA microarray analysis of MEF cells reveals common targets of menin and SUV39H1. Commonly upregulated genes by depletion of menin (Men−/−/Men+/+) and SUV39H1 (SUV39H1 siRNA/control) were indicated. Expression of GBX2 was measured using qRT-PCR in Men1+/+ or Men1−/− MEFs (b) as well as in MEFs that had been treated with Men1 siRNA (c). (d) The level of GBX2 mRNA was analyzed in Men1−/− MEFs infected with retroviral vectors expressing empty (control) or menin. (e) Knockdown of SUV39H1 increased the mRNA level of GBX2 in Men1+/+MEF cells, but not in Men1−/− MEF cells. Men1+/+ and Men1−/− MEF cells were treated for 1 day with siRNA specifically targeting SUV39H1 and total RNA was isolated to detect the steady state level of GBX2 by qRT-PCR. (f) Double knockdown of both menin and SUV39H1 did not have an additive effect on GBX2 mRNA level. The PCR values were normalized for GAPDH and presented as relative values by considering GBX2/GAPDH of control as 1. Error bars represent S.D., n=3. Significance of differences was evaluated (*P-value<0.05, **P-value<0.005)