Skip to main content
. 2013 Apr 11;4(4):e583. doi: 10.1038/cddis.2013.98

Figure 5.

Figure 5

Menin recruits SUV39H1 to promote trimethylation of H3K9 around the GBX2 locus. (a) GBX2 locus showing regions subjected to ChIP analysis. (b and c) Recruitment of menin and SUV39H1 along the GBX2 locus was analyzed by ChIP assay. Chromatin solution was prepared from Men1+/+ and Men1−/− cells and subjected to ChIP using control rabbit IgG and the antibodies against endogenous menin (anti-menin Ab) (b) or SUV39H1(anti-SUV39H1 Ab) (c). Immunoprecipitated DNA was analyzed in duplicates by quantitative real-time PCR with primers shown above (a) and the relative enrichment of proteins was shown. (d–f) ChIP assay with Men1+/+ and Men1−/− MEFs using antibodies against indicated H3 modifications was performed to measure the relative levels of H3 K4 or H3 K9 di-, trimethylation, and acetylation along the GBX2. The data represent the percentage of ChIP (IP/input) and error bars indicate the S.D., n=3. Significance of differences was evaluated (*P-value<0.05)