Figure 3.

Effects of autophagy inhibition in MCF7 cells subjected to PI. (a) Autophagy initiation or autophagy completion were inhibited using 10 mM of 3-methyladenine or 5 μM of chloroquine, respectively, and the expression of the pro-apoptotic transcription factor CHOP was analyzed in MCF7 cells. Note the very low expression of CHOP in cells cotreated with MG132 and 3-MA. (b) Cell viability assay in Atg5 knockdown MCF7 cells. Control siRNA cells, or control siRNA cells treated with 1 μM MG132 for 24 h, or Atg5 siRNA cells, or Atg5 siRNA cells treated with 1 μM MG132 for 24 h, were subjected to SRB cell viability assay. Data are presented as mean±S.D. of four independent experiments done in parallel. *P<0.05, significant difference compared with control condition. ^#P<0.05, significant difference compared with control MCF7 cells treated with MG132 1 μM