Figure 1.

The 12/15-LO inhibitor BA ameliorated EAE severity. (a and b) Clinical scores of EAE mice subjected to vehicle or BA treatment with the preventive (a) and treatment (b) protocols. Results are shown as mean±S.E.M. (n=6 mice per group) and representative of three independent experiments with similar results. (c and d) EAE mice were subjected to vehicle or BA treatment with the preventive protocol. Spinal cord sections were stained with H&E (left, top) or LFB (left, bottom) on day 20 post immunization. Original magnification, × 40. Cellular infiltration in H&E sections and demyelinated areas in LFB-stained sections were summarized as mean±S.E.M. (n=6) on the right. Scale bars, 500 μm (c). Flow cytometry were performed to analyze infiltration of CD45⁺CD4⁺ and CD45⁺CD8⁺ T cells in the spinal cords on day 10 post immunization (d). Results in c (left) and d are representative of three independent experiments. *P<0.05; **P<0.01