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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Eur J Immunol. 2012 Aug 6;42(10):2683–2696. doi: 10.1002/eji.201142317

Fig. 1. Enhanced induction of FoxP3+ iTregs in the presence of P4.

Fig. 1

(A) Treg-depleted naïve CD4+ T cells were cultured for 6–7 days in the presence or absence of P4 (2 μg/ml) and TGFβ1. Concanavalin A (2.5 μg/ml) and IL2 (100 U/ml) were added. (B) Induction of P4-iTregs by activation with anti-CD3/28 antibodies or antigen peptide (OVA323–339). Wild type C57BL/6 Treg-depleted naïve CD4+ T cells were activated with anti-CD3/28 antibodies, and DO11.10 Rag2(−/−) naïve CD4+T cells were activated with the OVA peptide and irradiated splenocytes. (C) Dose-dependent induction of FoxP3+ T cells in response to P4. The cells were cultured in the presence of TGFβ1 and IL2 and activated with anti-CD3/28 antibodies for 6 days. (D) Induction of LAP-TGFβ1 by P4 on T cells. TGFβ1 was used at 0.3 ng/ml for panel B and D. Representative or combined data (n=3–8/group) obtained from at least 3 independent experiments are shown. *Significant differences between control and P4 groups.