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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Eur J Immunol. 2012 Aug 6;42(10):2683–2696. doi: 10.1002/eji.201142317

Fig. 2. P4 induces iTregs with potent suppressive activity but does not induce demethylation in the Treg-specific demethylated region (TSDR) in the FoxP3 gene.

Fig. 2

(A) P4-induced iTregs were highly suppressive in vitro. Suppression of target T cell (CD4+CD25− responder) proliferation stimulated with anti-CD3 antibodies and irradiated splenic APCs for 3 days by Tregs was assessed based on dilution of CFSE fluorescence. (B) Methylation of the CpG sites in the TSDR region of nTregs, control Tregs and P4-Tregs. The iTregs were generated by culturing Treg-depleted naïve CD4+ T cells for 6 days in the presence or absence of P4 (2 μg/ml or 6.4 μM) and TGFβ1. Concanavalin A (2.5 μg/ml) and IL2 (100 U/ml) were used for T cell activation. Representative data obtained from 3 (panel A) and 2 (panel B) independent experiments are shown.