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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Eur J Immunol. 2012 Aug 6;42(10):2683–2696. doi: 10.1002/eji.201142317

Fig. 6. P4-induced iTregs are more effective than control iTregs in suppressing EAE development.

Fig. 6

(A) In vivo regulatory function of P4-induced antigen (MOG)-specific Tregs in regulation of EAE (n=17–21/group). (B) Cumulative clinical scores are the sums of all EAE clinical scores divided by the number of mice per group. (C) H&E histology of the spinal cords of the EAE mice treated with no Tregs, control (TGFβ1), or P4-iTregs. A representative image (n=17–21/group) from normal unimmunized mice without inflammation is also shown as a negative control. (D) P4-iTregs are effective in suppression of emergence of inflammatory T cells at an early time point (day 13). Absolute numbers of effector T cells from each tissue/organ are shown. The iTregs were prepared with a MOG peptide and irradiated splenocytes from 2D2 mice (A) or MOG-immunized mice (D) as described in the materials and methods section. Data from two independent experiments were pooled. Significant differences between indicated groups,* from control mouse groups,* or from control iTreg groups.**