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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Eur J Immunol. 2012 Aug 6;42(10):2683–2696. doi: 10.1002/eji.201142317

Fig. 8. P4-induced iTregs are more stable than control iTregs in FoxP3 expression.

Fig. 8

(A) Treg stability in vitro. Treg-depleted naïve CD4+ T cells were cultured for 6–7 days with concanavalin A in the presence of TGFβ1 (2 ng/ml) for control-iTregs or with TGFβ1 and P4 (2 μg/ml or 6.4 μM) for P4-iTregs. The T cells were re-cultured in the absence or presence of P4 and examined for FoxP3 expression. Representative data obtained from 5 independent experiments are shown. (B) Treg stability in vivo in an inflammatory condition. Frequencies of iTregs in the spleen and draining lymph nodes of EAE mice 28 days after the cell transfer are shown. A representative set of dot plot data and combined data (n=9–10; pooled from 2 independent experiments) in a graph form are shown.