Table 2. (d)CAPS markers used for identification of ACCase mutant alleles in wild-oat populations. Ambiguity nucleotide codes: R=A/G, Y=C/T.

Target mutation	Primer pair used	Type	Restriction enzyme	Recognition site	Fragment size (bp)
					Susceptible (S)	Resistant (R)
1781	NsiI1781F/ NsiI1781R	dCAPS	NsiI	5′-ATGCA^T-3′	130, 35	165
2078	RsaI2078F/ RsaI2078R	dCAPS	Rsal	5′-GT^AC-3′	181	147, 34
2088	SQCT-α1 F/ SQCT-α1 R	CAPS	HaeII	5′-RGCGC^Y-3′	602	529,73

Abbreviations: ACCase, acetyl-coenzyme A carboxylase; dCAPS, derived cleaved amplified polymorphic sequence.