Table 4. dCAPS and CAPS marker segregation of single-plant-derived (self-pollinated) progeny of the R2 population.
| Mutation(s) identified in parents | Progeny lines analyzed | No. of progeny plants |
Marker-identified genotypes in progeny
|
Inferred genotypes of the parents |
Phenotypic response to diclofop
a
|
||
|---|---|---|---|---|---|---|---|
| RS (mutation present) | S (mutation absent) | Resistant | Susceptible | ||||
| 1781 | 1 | 21 | 21 | 0 | AA_ _ _ _ | 21 | 0 |
| 2 | 18 | 18 | 0 | 18 | 0 | ||
| 2078 | 1 | 24 | 24 | 0 | BB_ _ _ _ | 24 | 0 |
| 2 | 20 | 20 | 0 | 20 | 0 | ||
| 2088 | 1 | 18 | 18 | 0 | CC_ _ _ _ | 18 | 0 |
| 2 | 18 | 18 | 0 | 18 | 0 | ||
| 1781+2088 | 1 | 24 | 24 (1781) | 0 | AACC _ _ | ||
| 24 (2088) | 0 | ||||||
| 2078+2088 | 1 | 32 | 32 (2078) | 0 | BBC_ _ _ | ||
| 27 (2088) | 5 | ||||||
| 1781+2078 +2088 | 1 | 71 | 55 (1781) | 16 | A_ B _ C _ | ||
| 50 (2078) | 21 | ||||||
| 49 (2088) | 22 | ||||||
Abbreviations: dCAPS, derived cleaved amplified polymorphic sequence; RS, resistant mutant and wild-type bands; S, the susceptible wild-type band.
Designations of R or S refer to the analyzed mutation-site nucleotides only. A=1781, B=2078 and C=2088 mutation, respectively.
a
When the genotyping finished, these plants with more than one mutation had already passed the 3- to 4-leaf stage and, therefore, were not treated with diclofop for phenotype assessment.