Table 5. Exact test for goodness of fit of observed (d)CAPS marker segregation to a 27:9:9:9:3:3:3:1 ratio expected from disomic inheritance of three unlinked gene loci vs a 147:69:69:69:15:15:15:1 ratio expected from polysomic inheritance of six randomly segregating gene loci, assuming that there is no chromatid segregation (no double reduction).
| Mutation/allele |
Disomic model
|
Polysomic model
|
||||||
|---|---|---|---|---|---|---|---|---|
| Genotype |
Progeny plants
|
Genotype |
Progeny plants
|
|||||
| if A=1781, B=2078, C=2088 | Observed | Expected | P | if A=1781, B=2078, C=2088 | Observed | Expected | P | |
| 1781+2078+2088 | A_B_C_ | 27 | 29.9 | ABC_ _ _ | 27 | 26.1 | ||
| 1781+2078 | A_B_cc | 12 | 9.9 | AB_ _ _ _ | 12 | 12.2 | ||
| 1781+2088 | A_bbC_ | 12 | 9.9 | AC_ _ _ _ | 12 | 12.2 | ||
| 2078+2088 | aaB_C_ | 8 | 9.9 | BC_ _ _ _ | 8 | 12.2 | ||
| 1781 only | A_bbcc | 5 | 3.3 | A_ _ _ _ _ | 5 | 2.7 | ||
| 2078 only | aaB_cc | 4 | 3.3 | B_ _ _ _ _ | 4 | 2.7 | ||
| 2088 only | aabbC_ | 1 | 3.3 | C_ _ _ _ _ | 1 | 2.7 | ||
| Nil | aabbcc | 2 | 1.1 | wwwwww | 2 | 0.2 | ||
| Total | 71 | 71 | 0.66 | 71 | 71 | 0.009 | ||
Abbreviation: dCAPS, derived cleaved amplified polymorphic sequence.
Observations were made among progeny plants derived from a self-pollinated parent plant containing 1781, 2078 and 2088 mutations (A, B and C, respectively). In the disomic model, the parent would be heterozygous for each mutation (AaBbCc × AaBbCc). In the polysomic model, the parent would have all three mutations plus three wild-type alleles (ABCwww × ABCwww). Wild-type alleles are designated a, b or c in the disomic model and w in the polysomic model, and P is probability.