TABLE 1.
Organism | Protein | Accession no. | Size (amino acids) | Maximum % identity (similarity)b | Storagec | Activity in E. colid |
Reference | |
---|---|---|---|---|---|---|---|---|
WS | DGAT | |||||||
A. baylyi ADP1 | AtfA | AAO17391 | 458 | 100 (100) | WE, TAG | ✓ | ✓ | 51 |
M. tuberculosis H37Rv | Tgs1 | NP_217646 | 463 | 27 (44) | TAG | ✓ | 73 | |
Tgs2 | NP_218251 | 454 | 38 (58) | (✓) | ✓ | |||
Tgs3 | NP_217751 | 271 | 29 (47) | ✓ | ||||
Tgs4 | NP_217604 | 474 | 29 (47) | ✓ | ||||
M. hydrocarbonoclasticus | WS1 | ABO21020 | 455 | 46 (66) | (ip) WE | ✓ | ✓ | 32 |
WS2 | ABO21021 | 473 | 39 (60) | ✓ | ||||
A. borkumensis SK2 | AtfA1 | YP_694462 | 457 | 50 (69) | TAG (WE) | ✓ | ✓ | 26 |
AtfA2 | YP_693524 | 451 | 41 (60) | ✓ | ||||
R. opacus PD630 | Atf1 | ACX81314 | 462 | 25 (43) | TAG (WE) | ✓ | 74 | |
Atf2 | EHI41112 | 453 | 39 (61) | ✓ | ✓ | |||
S. coelicolor A3(2) | Sco0958 | NP_625255 | 446 | 25 (42) | TAG | ✓ | 75 | |
Sco1280 | NP_625567 | 413 | 22 (34) | (✓ ?) | ||||
S. avermitilis MA-4680 | SAV7256 | NP_828432 | 447 | 25 (41) | TAG | ✓ | (✓) | 76 |
P. arcticus 273-4 | DGAT | YP_263530 | 475 | 52 (72) | ? | ✓ | ? | 72 |
P. hybrida | PhWS1 | AAZ08051 | 521 | 22 (40) | WE | ✓ | 53 | |
A. thaliana | WSD1 | AED94163 | 481 | 22 (41) | WE | ✓ | (✓) | 13 |
Abbreviations: WE, wax esters; TAG, triacylglycerols; (ip) WE, isoprenoid wax esters; WS, wax synthase; DGAT, acyl-CoA:diacylglycerol acyltransferase activity.
Maximal percentage of identical or similar amino acids compared to AtfA from A. baylyi strain ADP1.
Natural function of the respective enzyme in its native host; parentheses indicate that WE were synthesized only when precursors (such as hexadecanol) were supplied.
Enzymatic activity of the respective enzyme (catalysis of WS and/or DGAT reactions) upon heterologous expression in E. coli.