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. Author manuscript; available in PMC: 2013 May 31.
Published in final edited form as: Cell Microbiol. 2008 Jan 31;10(6):1286–1303. doi: 10.1111/j.1462-5822.2008.01126.x

Fig. 6.

Fig. 6

ΔfbpA phagosomes efficiently acquire both early and late endosomal markers when compared with H37Rv and BCG within MΦs. MΦs were labelled with lysotracker red (LTR), mannosylated BSA rhodamine (MBR), or transferrin Texas red (TRR), chased with medium and infected with gfpΔfbpA, gfpBCG or gfpH 37Rv strains. MΦs were washed and examined for fluorescence colocalization.

A. LSM profile illustrates that live gfpΔfbpA has markedly enhanced colocalization with LTR while the wild-type gfpH 37Rv excludes LTR.

B. Per cent phagosomes positive for colocalization was determined by using LSM in three separate experiments. gfpΔfbpA significantly colocalizes with both LTR and MBR compared with either gfpBCG or gfpH 37Rv (Student's t-test). Colocalization with TRR, an early endosomal fusion marker, was comparable for all three strains tested. Scoring was carried out as in Fig. 4.