Table I.
Plasmids used in this study
| Plasmid | Origin, Insert | Marker | Backbone |
|---|---|---|---|
| pJPA105 | 2 μ, rDNA repeat (XmaI end points) | TRP1 | pAF15a |
| pJPA106 | 2 μ, rDNA repeat (AhdI end points) | TRP1 | pAF15 |
| pJPA107 | 2 μ, rDNA repeat (PsiI end points) | TRP1 | pAF15 |
| pJPA113 | ARS1 | URA3 | pRS306 (43) |
| pJPA114 | rDNA ARS | URA3 | pRS306 |
| pJPA116 | ARS1, CEN4 | URA3 | pRS306 |
| pJPA117 | rDNA ARS, CEN4 | URA3 | pRS306 |
| pJPA138 | 2 μ | URA3 | pRS306 |
| pJPA133 | ARS1 | LEU2 | pRS305 (43) |
| pJPA136 | ARS1, CEN4 | LEU2 | pRS305 |
| pJPA148 | 2 μ | LEU2 | pRS305 |
s
Derived from pRS424 (8), see “Experimental Procedures.”