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. Author manuscript; available in PMC: 2013 May 31.
Published in final edited form as: Cell Host Microbe. 2010 Jul 30;8(2):137–146. doi: 10.1016/j.chom.2010.07.002

Figure 2. DAG production on SCVs is independent of autophagy. (a-b).

Figure 2

Wild-type (WT) and autophagy-deficient (atg5 −/−) MEFs were transfected with either PKCδ-C1-GFP (a) or GFP-LC3 (b). Cells were infected with S. Typhimurium expressing mRFP (RFP-Sal). Cells were fixed at 45 min a or 1 h b p.i. Representative confocal z-slices are shown. The inner panels represent a higher magnification of the boxed areas. Size bar, 10 μm. (c) WT and atg5−/− MEFs were transfected and infected as in a-b and fixed at the indicated time-points. PKCδ-C1-GFP+ or GFP-LC3+ RFP-Sal were determined by fluorescence microscopy. Data represent the mean ± standard error (s.e.m.) for at least three independent experiments.