FIGURE 2.

Expression of GARP is gained in the thymus upon expression of Foxp3. (A) Staining of GARP on CD4⁺Foxp3⁺ cells from pooled lymph nodes (left panel) or CD4⁺CD8⁻Foxp3⁺ cells from the thymus of 6 week old C57BL/6 mice. (B) CD4^SPFoxp3⁻CD25^hi cells were sorted from 6–8 week old Foxp3-GFP knock-in mice, and then cultured overnight without supplementing any cytokines or supplementing IL-2 (100U/ml). (C) Staining for GARP gating on CD4⁺Foxp3⁻ and CD4⁺Foxp3⁺ cells from IL-2 cultured CD4^SPFoxp3⁻CD25^hi thymocytes from (B).