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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Immunol. 2013 May 3;190(11):5506–5515. doi: 10.4049/jimmunol.1300199

FIGURE 5.

FIGURE 5

Neither GARP nor TGF-β1 contributes to Treg suppression. (A) Wild type T effector (CD4+CD25−, 5x104) cells were activated with soluble anti-CD3 and irradiated T-cell depleted splenocytes in the presence or absence of wild type or GARP−/− Tregs from lrrc32fl/fl x CD4-CRE mice at an effector to Treg ratio of 16:1 to 1:2 for 3 days. Cultures were pulsed with [3H]-thymidine for the last 6 hours of culture. (B) As in (A), except using wild type or TGF-β1−/− Tregs from tgfb1fl/fl x CD4-CRE mice. (C) Wild type or GARP−/− Tregs from lrrc32fl/fl x CD4-CRE were stimulated with soluble anti-CD3 and irradiated T-cell depleted splenocytes in the presence or absence of IL-2. Cultures were pulsed as in (A) after 3 days.