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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Immunol. 2013 May 3;190(11):5506–5515. doi: 10.4049/jimmunol.1300199

FIGURE 7.

FIGURE 7

Treg-mediated Th17 differentiation and Treg induction require Treg derived TGF-β1 and presentation via GARP. Naïve CD45.1+ OT-II cells from Rag1−/− mice were activated with soluble anti-CD3 (1μg/ml) and dendritic cells in the presence of IL-6 (10ng/ml) and pre-activated CD4+CD25hi Tregs from tgfb1fl/fl x CD4-CRE (A), furinfl/fl x CD4-CRE (B), and lrrc32fl/fl x CD4-CRE (C) mice as in Figure 6. Cells from CRE-negative littermates were used as controls. Percents indicate the percentage IL-17A+ from the CD4+CD45.1+CD45.2− cells within the culture. Bar graphs on right panels indicate the average of duplicates within the experiment ± SD. (D) Naïve CD45.1+ OT-II cells from Rag1−/− mice were activated with soluble anti-CD3 (1μg/ml) and dendritic cells in the presence of IL-2 (100U/ml) and pre-activated CD4+CD25hi Tregs from lrrc32fl/fl x CD4-CRE.