TABLE 1.
Surface plasmon resonance experiments for HLA-B35LPEP and the CA5, SB27, and SB47 TCRs
The experiments were conducted at least twice (n = 2) in duplicate and the values represent the mean ± S.E.
| Immobilized TCR | Analyte | Kdeq | Kon | Koff | t½a | Kdcalc |
|---|---|---|---|---|---|---|
| μm | × 104m−1 s−1 | s−1 | s | μm | ||
| CA5 | HLA-B*35:08LPEP | 3.75 ± 0.01 | 50.80 ± 8.06 | 0.19 ± 0.02 | 3.62 ± 0.32 | 3.78 ± 0.95 |
| SB27 | HLA-B*35:08LPEP | 12.15 ± 0.35 | 10.05 ± 0.92 | 0.11 ± 0.01 | 6.21 ± 0.55 | 11.14 ± 0.03 |
| SB47 | HLA-B*35:08LPEP | 25.00 ± 0.28 | 15.05 ± 5.16 | 0.30 ± 0.01 | 2.33 ± 0.05 | 19.77 ± 6.75 |
| CA5 | HLA-B*35:01LPEP | 27.10 ± 0.42 | 34.45 ± 6.01 | 0.67 ± 0.16 | 1.07 ± 0.26 | 19.38 ± 1.34 |
| SB27 | HLA-B*35:01LPEP | 52.25 ± 4.88 | NDb | NDb | NDb | NDb |
| SB47 | HLA-B*35:01LPEP | 29.25 ± 0.07 | 15.50 ± 0.03 | 0.33 ± 0.04 | 2.15 ± 0.24 | 20.9 ± 1.99 |
a t½ = 0.693/Kdeq.
b ND, not determined.