FIGURE 3.

Colchicine-induced myofibroblast differentiation is mediated by ROCK and SRF activities. HLF were pretreated for 30 min with or without 10 μm Y27632 or 10 μm CCG-1423 as indicated, followed by stimulation with 1 μm colchicine (Colch) for 24 h. A, colchicine stimulates phosphorylation of cofilin, as assessed by Western blotting with phosphor-Ser-3-cofilin antibodies. B, Rho kinase inhibition by Y27632 attenuates colchicine-induced actin stress fiber formation and α-SMA protein expression. C, the SRF inhibitor, CCG-1423, blocked colchicine-induced α-SMA protein expression. Densitometry results are shown below the respective blots.