FIGURE 4.

TGF-β1 does not regulate gross microtubule polymerization state. A, immunofluorescent staining of HLF with α-tubulin antibodies following 24-h stimulation with TGF-β1. Scale bar, 100 μm. B and C, HLF were pretreated with either vehicle, 10 μm paclitaxel, or 1 μm colchicine (Colch) for 30 min, followed by stimulation with 1 ng/ml TGF-β1 for either 1 (B) or 24 h (C). Microtubule and soluble tubulin fractions were isolated and examined by Western blotting with α-tubulin antibodies. Densitometry results are shown below the respective blots.