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Polymerization of microtubules by paclitaxel attenuates TGF-β1-induced myofibroblast differentiation. HLF were pretreated with indicated concentration of paclitaxel for 0.5 h, followed by stimulation with 1 ng/ml TGF-β1 for 24 h. Cell extracts were examined for the expression of SRF, CTGF, and α-SMA mRNA by real time quantitative PCR (A) or of protein levels by Western blotting (B).
