FIGURE 6.

Paclitaxel regulates TGF-β1-induced stress fiber formation, MKL1 nuclear accumulation, and SRF activation. A, HLF were pretreated with 10 μm paclitaxel for 0.5 h, followed by stimulation with 1 ng/ml TGF-β1 for 24 h. Cells were analyzed for stress fiber formation by fluorescent phalloidin staining. Scale bar, 200 μm. Under these treatment conditions, HLF were also assessed for total α-SMA expression and stress fiber formation by in vitro fractionation (B), for nuclear accumulation of MKL1 by in vitro fractionation (C), or for SRF-luciferase reporter activation (D).