FIGURE 7.

TGF-β1-induced accumulation of mDia2 with stress fibers and regulation by microtubule dynamics. Co-localization of mDia2 with microtubules in quiescent fibroblasts, as assessed by immunofluorescent microscopy are shown (A and B). A, shown are the images of HLF stained with antibodies against α-tubulin, mDia2, or merged (counter stained with DAPI) as indicated, taken at 600× magnification. Scale bar, 50 μm. B, the same fields as in A were imaged at 1000× magnification, and the selected areas (dashed lines) were magnified using Adobe Photoshop. Scale bar, 10 μm. C, HLF were pretreated with or without 10 μm paclitaxel for 0.5 h, followed by stimulation with 1 ng/ml TGF-β1 for 24 h, as indicated. mDia2 levels in stress fiber fraction as well as total levels of mDia2 were examined by Western blotting with mDia2 antibodies. Densitometry results are shown below the respective blots. Colch, colchicine.