FIGURE 8.
mDIA activity is required for TGF-β1-induced actin stress fiber formation and myofibroblast differentiation. A–D, HLF were pretreated with indicated concentrations of SMFH2 for 30 min, followed by treatment with 1 ng/ml TGF-β1, 10 μm paclitaxel, or 1 μm colchicine (Colch) for the times indicated. Cell extracts were examined by Western blotting for the expression of α-SMA (A), stress fiber formation (B), phosphorylation of Smad2 (C), or microtubule polymerization state (D). Densitometry results are shown below the respective blots.
