FIGURE 2.

CP55940-induced increased GRK5 and reduced GRK2 expression levels in CLU213 cells. Cells were incubated with either vehicle (ethanol, 0.01% final concentration) or CP55940 (1 nm) for 72 h. Cells were washed (three times) with PBS every 24 h, and fresh vehicle or CP55940 (1 nm) was added. A and B, Western blots to show alteration or no alteration in GRK5 (A), GRK6 (B), or GRK2 (C) protein levels after repeated CP55940 treatment. Proteins (8 μg) were resolved by SDS-PAGE as described under “Experimental Procedures.” D, qRT-PCR to assess GRK5, GRK6, and GRK2 mRNA levels. **, p < 0.01; *, p < 0.05, significant effect of CP55940 treatment compared with vehicle-treated controls. The data represent mean ± S.E. (error bars) (n = 3).