FIGURE 1.

Amino acid sequence alignment of Paenibacillus macerans licheninase (PDB code 1u0a), P. trichocarpa endoglucanase 16 (PtEG16, POPTR_0002s15460), P. tremula x tremuloides xyloglucan endotransglycosylase 16-34 (PttXET16-34, PDB code 1umz), and Tropaeolum majus xyloglucan endotransglycosylase (TmNXG1, PDB code 2uwa) of GH16. The alignment was performed with MUSCLE (25), and secondary structure elements (strands (arrows), helices (spirals), and turns (T)) from the corresponding crystal structures were added with ESPript (83) (protein names are in italic type). Conserved catalytic residues are marked with asterisks.