FIGURE 3.

Time-dependent endohydrolytic cleavage of polysaccharides by PtEG16. The action of PtEG16 on barley β-glucan (A), Icelandic moss lichenin (B), and tamarind seed galactoxyloglucan (C) was analyzed using high performance size exclusion chromatography with evaporative light-scattering detection (ELS). The t = 0 trace corresponds to that of the corresponding polysaccharide before the addition of the enzyme. Compositional analyses of the oligosaccharide mixtures resulting from overnight incubation are shown in supplemental Figs. S3–S5. Peaks marked with an asterisk arise from low components the enzyme preparation (e.g. buffer and salts).