TABLE 2.
Properties of AcnR derivatives used
| Mutation | Location | Further information |
|---|---|---|
| pEKEx2-AcnR-STREP derivatives | ||
| K43A | DBD | Almost complete abolishment of DNA binding |
| K55A | DBD | Complete abolishment of DNA binding |
| K104A | Dimer interface | DNA affinity close to wild-type protein |
| E65A | Beginning of α4 | Maybe involved in signal transduction to the DNA binding domain, no effect on DNA binding |
| D66A | Beginning of α4 | Maybe involved in signal transduction to the DNA binding domain, no effect on DNA binding |
| R99A | P pocket | Weaker binding to DNA |
| D109A | Protein surface | No effect on DNA binding |
| R130A | D pocket | Hydrogen-bonds to citrate, does not change its affinity to DNA in the presence of citrate-Mg2+ |
| R141A | Protein surface | No effect on DNA binding |
| D143A | Protein surface | No effect on DNA binding |
| D158A | P pocket | Weaker binding to DNA |
| E181A | D pocket | Binds Mg ion, does not change its affinity to DNA in the presence of citrate-Mg2+ |
| R185A | D pocket | Hydrogen-bonds to citrate, does not change its affinity to DNA in the presence of citrate-Mg2+ |
| pET-TEV-AcnR derivatives | ||
| C23A | Within α1 | No obvious difference in behavior, |
| R69A | P pocket | No obvious difference in behavior |
| M70A | P pocket | No obvious difference in behavior |
| M86A | P pocket | No obvious difference in behavior |
| R92A | P pocket | No obvious difference in behavior |
| W95A | P pocket | No obvious difference in behavior |
| R130A | D pocket | Does not change its affinity to DNA in the presence of citrate-Mg2+ |