FIGURE 8.

Results from the Morris water maze tests (n = 8). Repeated measures analysis of variance revealed statistically significant differences (p < 0.01) (day/group interaction) between the HI, shRNA, and cDNA groups but did not reveal differences between the saline or ADV-null vector injection groups (A). ▾, Cygb-shRNA-ADV injection + HI group; ●, HI group; □, saline injection + HI group; ■, ADV vectors only + HI group; ▴, Cygb-cDNA-ADV injection + HI group; ○, sham group. On the first day, the EL values of these groups were narrow in range. On the fourth day, the EL values of the shRNA group were higher as compared with the HI group; however, EL values in the Cygb-cDNA injection group were much lower than those in the HI group (p < 0.01). On the fifth day, the sham group had a short swim path to the former platform location, whereas the rats of the other groups had longer swim paths. The platform crossing (swimming into the area where the former platform was located within 90 s after removing the platform) was lower in the HI, saline, and ADV only groups compared with the sham group (p < 0.01). B, the number of platform crossings in the shRNA injection group was lower compared with the HI model group (p < 0.05) and also significantly less compared with the cDNA injection group (p < 0.01). C, comparison of the percentage of time spent in the target quadrant among different groups. a, p < 0.01 as compared with the sham group. b, significant difference; c, p < 0.01 as compared with the HI group. Error bars, S.D.