FIGURE 10.
Endocytosis of the (DSS)6- FLAG peptide. A and B, representative three-dimensional z-stack reconstructions of confocal images show that the FLAG antibody bound non-specifically to the cell surface and the cover glass when MC3T3 cells were incubated with it at 37 °C for 1 h (A) and that it is present in the cytoplasm when pre-bound to the (DSS)6-FLAG peptide. Images A and B show the reconstruction in the x-y plane, and images A1 and B1 show the reconstruction in the x-z plane. White arrows in A1 point to nonspecific binding of the FLAG antibody to the cell surface and to the cover glass. Yellow arrows in B1 point to intracellular presence of the antibody.