FIGURE 2.

MES phosphorylates p53 transiently and cumulatively in several epithelial cell lines. A, HCT116 cells were treated with MES (0.1 ms) for 10 min, and protein lysates were isolated at 0, 1, 3, or 6 h after treatment. p-p53, phospho-p53. B, cells were treated with MES (0.1 ms) for 0, 10, 20, or 30 min, and protein lysates were recovered 1 h after treatment. C, HCT116, A549, HEK293, and HepG2 cells were treated with MES (0.1 ms) for 10 min, and lysates were extracted 1 h after treatment. con, control. For A–C, cell lysates were analyzed by immunoblotting with the indicated antibodies. Actin served as an internal control.