FIGURE 2.

Reprogramming of pancreatic acinar cells induced by AdAmy2TRα. mRNA levels for 11 genes associated with the differentiation of β-cells were assayed in AdAmy2TRα-infected acinar cells treated with 100 nm T3. From four independent samples, total RNA was isolated. The amounts of insulin1, insulin2, glucokinase, amylase2, Hnf6, Foxa2, Nkx2.2, NeuroD1, Pdx1, Ngn3, and Mafa mRNA were determined by quantitative real-time RT-PCR with 100 ng of cDNA in triplicate. Relative quantification of target cDNA was determined by arbitrarily setting the control value from untreated samples to 1. All data are expressed as the mean ± S.D. (error bars). *, p < 0.05 compared with untreated cells.