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Truncating the C terminus of p51 HIV-1 RT increases thermal stability of reconstituted selectively deleted p66/p51 heterodimers. Differential scanning fluorometry was performed in the absence of divalent metal or in the presence of 10 mm MgCl2 or 0.5 mm MnCl2 as indicated. Results are the average of duplicate analysis. p51 deletions Δ5, Δ9, and Δ13 remove C-terminal residues Gly-436–Phe-440, Glu-432–Phe-440, and Gln-428–Phe-440, respectively.
