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. 2013 May 24;123(6):2421–2433. doi: 10.1172/JCI65952

Figure 2. Analysis of Osteocalcin–/– male mouse fertility after hCG or osteocalcin injection.

Figure 2

Sperm count (A); testis (B), epididymal (C), and seminal vesicle (D) weights normalized to BW (mg/g of BW), (E) circulating testosterone levels in WT and Osteocalcin–/– mice at 10 weeks of age, both injected either with PBS (WT, n = 20; Osteocalcin–/–; n = 14; Gprc6a–/–, n = 7), hCG (WT, n = 12; Osteocalcin–/–, n = 8; Gprc6a–/–, n = 6) (5 UI, twice a week), or osteocalcin (WT, n = 6; Osteocalcin–/–, n = 6; Gprc6a–/–, n = 6) (3 ng/g/d). All analyses were performed on nonbreeder 129/Sv mice. Error bars represent SEM. *P < 0.05, Student’s t test. Specifically, P < 0.05 versus WT injected with hCG (5 UI, twice a week); and P < 0.05 versus WT injected with osteocalcin (3 ng/g/d).