Figure 1. Resistance of Tnfrsf1a^+/– mice to TNF-induced lethal inflammation.

(A) Serum IL-6 6 hours after a single i.p. injection of 100 μg TNF in the Rothe (12), Pfeffer (14), and Peschon (13) Tnfrsf1a-deficient mouse lines. ***P < 0.001, compared with Tnfrsf1a^+/+. (B) Body temperature (***P < 0.001, compared with Tnfrsf1a^+/– and Tnfrsf1a^–/–), (C) sickness score, (D) serum AST and ALT levels, and (E) intestinal permeability of Tnfrsf1a^+/+, Tnfrsf1a^+/–, and Tnfrsf1a^–/– mice after i.p injection with 50 μg TNF i.p. (or 25 μg i.p. for permeability assay) (n = 8 for all groups). (F) p55TNFR expression, measured by ELISA, in liver samples. (G) Specific binding of ¹²⁵I-hTNF to BMDMs. In D and F, levels in Tnfrsf1a^–/– and Tnfrsf1a^+/– were 0, so no statistical significance could be calculated toward Tnfrsf1a^+/+ data. Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test).