Figure 3. Antimicrobial effects, immune response, and spleen germinal centers in Tnfrsf1a^+/– mice.

(A) Survival of mice following i.p. challenge with 10⁵ Listeria monocytogenes per mouse. (B) Germinal center formation and follicular dendritic cell networks in spleens of mice immunized with SRBCs are evident after staining with either PNA (blue) or CR-1 (brown). In Tnfrsf1a^+/+ and Tnfrsf1a^+/– mice, germinal centers and follicular dendritic cell networks are both detected, but they are absent in Tnfrsf1a^–/– mice. Original magnification, ×40. (C) Serum antibody responses to SRBC immunization. Data are expressed in arbitrary units as the mean ± SEM of at least 10 mice per group and are representative of 3 independent experiments. **P < 0.01, ***P < 0.001.