Skip to main content
. 2013 May 24;123(6):2703–2718. doi: 10.1172/JCI62980

Figure 1. Reduced ADAR1 expression is a common event in melanoma.

Figure 1

(A) Analysis of the immunohistochemical expression levels of ADAR1. Lines in the boxes denote the mean. (B) ADAR1 expression in low-passage metastatic melanoma cultures, as determined by qPCR. Results are expressed as fold above the average values in all normal melanocyte specimens. Cutoffs for overexpression and downregulation were determined as greater than 2 and less than 0.5, respectively. Data represent the mean ± SEM of 2 experiments on independent RNA purifications, each performed in triplicate. (C) ADAR1 expression at the protein level of selected low-passage melanoma cultures (samples A–G in part B), as determined by Western blot. A representative blot is shown. (D) ADAR1 expression at the protein level as determined by Western blot and (E) A-to-I editing rate of BLCAP by ADAR1 as determined by Sequenom MassArray in ADAR1-manipulated 624mel cell system: stable KD of ADAR1 (ADAR1-KD), rescue of ADAR1-P150 or ADAR1-P110 (rescue-P150, rescue-P110, respectively), and transfection with scrambled sequence and empty pcDNA3 (control). Data represent the mean ± SEM of 3 independent experiments, each performed in triplicate. **P < 0.01 (2-tailed t test, ANOVA); ***P < 0.0001 (ANOVA).