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. 2013 May 8;123(6):2643–2653. doi: 10.1172/JCI66735

Figure 2. Essential role of ET-2 in growth and survival of postnatal mice.

Figure 2

(A) qRT-PCR analysis demonstrating an absence of Et2 mRNA in individual tissues of constitutive Et2-null mice (n = 5). Values are presented as fold change relative to ET-2 expression in stomach from WT mice. (B) Body weight during pre-weaning period of constitutive Et2-null and littermate WT mice (n = 15). Inset shows a representative photograph of Et2-null (right) and WT (left) mice at 2 weeks of age. (C) Survival ratio of constitutive Et2-null and littermate WT mice (n = 100–102). (D) qRT-PCR analysis demonstrating an absence of Et2 mRNA level in individual tissues of neonatal and adult Et2f/f;CAGGCre-ERTM mice (n = 5). Values are presented as fold change relative to ET-2 expression in lung from adult Et2f/f control mice. ND, not detected. (E and F) Body weight (E) and survival ratio (F) of neonatal Et2f/f;CAGGCre-ERTM and littermate control Et2f/f mice (n = 9–17). (G) Body weight of adult Et2f/f;CAGGCre-ERTM and control littermate Et2f/f mice (n = 9–10). (H) Fat content of adult Et2f/f;CAGGCre-ERTM and littermate control Et2f/f mice after 10 weeks of tamoxifen (TAM) injection (n = 9–10). *P < 0.05; **P < 0.01; #P < 0.001.