Skip to main content
. 2013 May 8;123(6):2742–2751. doi: 10.1172/JCI67398

Figure 1. Murine TRLs require ApoE for binding to cell surface heparan sulfate.

Figure 1

(A) TRLs (δ < 1.006 g/ml) were isolated from fasted WT Ndst1f/fAlbCre– and mutant Ndst1f/fAlbCre+ mice (n = 4 per group) and analyzed by gradient SDS-PAGE. Individual proteins were visualized by silver staining. A representative gel is shown with n = 2 per genotype. (B) Binding of [3H]TRLs (50 μg/ml) from WT and Apoe–/– mice to Hep3B cells was measured before (filled bars) and after (open bars) treatment with heparin lyases (n = 5). (C) ApoE-deficient [3H]TRLs were reconstituted with recombinant human ApoE3 and purified by ultracentrifugation (δ < 1.006 g/ml). Cell surface binding of the reconstituted particles was measured (n = 8).