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. 2013 May 24;123(6):2366–2379. doi: 10.1172/JCI66451

Figure 4. Suppression of Pol II phosphorylation by E. coli 83972.

Figure 4

(A) Active Ser2 phosphorylated Pol II was stained using monoclonal primary human phospho CTD Ser2 antibodies and peroxidase-labeled secondary antibodies (brown). E. coli 83972 markedly suppressed Pol II phosphorylation compared with uninfected or CFT073-infected human kidney cells (A498). E. coli CFT073 had a nonsuppressive effect. (B) Inhibition by E. coli 83972 of Pol II phosphorylation in primary human kidney cells (HRTEC) compared with uninfected or CFT073-infected cells. (C) The specific Pol II inhibitor DRB (60 μM) abrogated Pol II phosphorylation in response to E. coli CFT073 compared with A498 cells without an inhibitor. For dose-dependent inhibition of Pol II phosphorylation in uninfected A498 cells, see Supplemental Figure 7A. (D) Competition between E. coli CFT073 and 83972 in A498 cells. E. coli 83972 inhibited Pol II phosphorylation in the presence of E. coli CFT073. (A and C) Scale bars: 50 μm and 10 μm (insets). #P = compared with medium control; ##P = compared with CFT073-infected cells; χ2 test for independence.