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. 2013 May 15;123(6):2654–2662. doi: 10.1172/JCI66301

Figure 5. Adoptive transfer of TRAIL+ T cells leads to suppression of alloactivated T cells.

Figure 5

(A) Purified T cells from B6 mice were injected into lethally irradiated CBF1 hosts. Single-cell suspension of splenocytes was analyzed by flow cytometry on day 4. DR5 MFI in CD25hi and CD25lo cells was analyzed in total donor T cells, CD4+ T cells, and CD8+ T cells. Representative data (n = 4 per group) from 2 independent experiments are shown. (B) Activated B6 T cells were used as targets of GFP+ and TRAIL+ T cells in a CTL assay. Graph representative of 2 independent experiments is shown. (C and D) Lethally irradiated CBF1 recipients were reconstituted with 5 × 106 cells per recipient of WT B6 TCD BM and 1 × 106 cells per recipient of B6 WT or DR5 KO B6 TRAIL+ T cells. Survival was monitored (C) and clinical GVHD scores were recorded weekly (D) in a blinded fashion. Graph representative of 2 independent experiments is shown. *P < 0.05; **P < 0.01; ***P < 0.001.