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. 2013 May 24;8:1935–1946. doi: 10.2147/IJN.S43074

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© 2013 Tong et al, publisher and licensee Dove Medical Press Ltd

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

PMC Copyright notice

Lineage identification and characterization of rat MSCs. (A) Expression of rat MSC markers (CD44 and CD90.1), endothelial cell marker (CD31), leukocyte marker (CD45), and hematopoietic stem cell marker (CD34) on rat BMSCs. (B) Alizarin Red S staining of rat BMSCs detected after culture for 21 days in osteogenic medium or basal medium. (C) Chondrogenic differentiation potential of the micromass of rat BMSCs in vitro. Alcian blue staining of cells was detected after culture for 14 days in chondrogenic medium or basal medium. (D) Adipogenic differentiation potential of rat BMSCs in vitro.

Note: Oil Red O staining of cells was detected after culture for 14 days in adipogenic medium or basal medium.

Abbreviations: MSC, mesenchymal stem cell; BMSCs, bone marrow mesenchymal stem cells.