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. 2013 May 25;13:259. doi: 10.1186/1471-2407-13-259

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Copyright © 2013 Zhou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MSP analysis of RASSF1A gene in ESCC and GCA tissue. Representative MSP results of three ESCC tissues (A, T1, T2, and T3) and (B) was the results of three GCA tissues (T4, T5, and T6). Lane M: indicates the presence of methylated genes; Lane U: indicates the presence of unmethylated genes. T1 and T4 were fully hypermethylation which revealed 93 bp band (M) with hypermethylated primers; T3 and T6 were unmethylation, having only unmethylated band of 105 bp; T2 and T5 were Hemi-methylation with both hypermethylated band and unmethylated band.