Table 1.
Identification of differentially expressed proteins of E coli DH5α treated with PySSPy, PyRSPy and 3A
| No. | ICBI accession No. | Protein identified | Calculated pI/MW (kDa) | Matched (Measured) peptide | Sequence coverage (%) |
Relative intensitiesa |
||
|---|---|---|---|---|---|---|---|---|
| PySSPy | PyRSPy | 3A | ||||||
| 1 |
GLYA_EC024 |
Serine hydroxymethyl-transferase |
6.03/94 |
9 (22) |
29 |
-25 ± 04 |
-2.1 ± 0.2 |
-1.4 ± 0.1 |
| 2 |
IR3Z2_ECOUT |
Glycerol kinase |
5.97/60 |
15 (30) |
33 |
-2.3±0.4 |
-1.6±0.05 |
-1.7±0.1 |
| 3 |
DHSB_ECQU |
Succinate dehydrogenase iron-sulfur subunit |
6.327 |
10 (21) |
47 |
-2.4 ± 0.1 |
-3.5 ± 0.3 |
-3.4 ± 0.1 |
| 4 |
KDUD_ECOLI |
2-dehydro-3-deoxy-D-gluconate 5-dehyrixenase |
5.24/27 |
8 (18) |
40 |
+1.7 ± 0.1 |
+2.0 ± 0.2 |
+1.9 ± 0.2 |
| 5 |
IvEH_ECO24 |
Malate dehydrogenase |
5.61/32 |
11 (22) |
49 |
+2.3 ± 0.2 |
+3.1 ± 0.05 |
+1.9 ± 0.06 |
| 6 |
CH6O1_ECOK1 |
60 kDa chaperonin 1 |
4.85157 |
10 (26) |
33 |
+2.4 ± 0.1 |
+2.5 ± 0.2 |
+3.1 ± 0.4 |
| 7 |
MAO2_ECOLI |
NADP-dependent malic enzyme |
5,3494 |
11 (13) |
14 |
-21 ± 0.5 |
-2.1±0.3 |
NS |
| 8 |
DPS_EC024 |
DNA protection during starvation protein |
5.72/19 |
9 (1) |
58 |
-2.3 ± 0.1 |
-2.8 ± 0.6 |
NS |
| 9 |
PCKA_EC024 |
Phosphoenolpyruvate carboxykinase |
5.46/60 |
14 (27) |
35 |
-24 ± 0.2 |
NS |
NS |
| 10 |
GCST_EC024 |
Aminomethyltransferase |
5.36/40 |
9 (17) |
35 |
-2.1 ± 0.3 |
NS |
NS |
| 11 |
SODF_ECO57 |
superoxide dismutase [Fe] |
5.58/53 |
15 (16) |
37 |
NS |
+1.9 ± 0.009 |
NS |
| 12 |
ATPB_EC024 |
ATP synthase subunit beta |
4.9150 |
16 (50) |
50 |
+3.0±0.2 |
NS |
+2.6 ± 0.2 |
| 13 |
EFTIJ1_EC024 |
Elongation factor Tu 1 |
53143 |
17 (44) |
53 |
+3.3 ± 0.2 |
NS |
+3.1 ± 0.1 |
| 14 |
TNAA_EC024 |
Tryptophanase |
5.88/53 |
15 (16) |
37 |
NS |
+1.9 ± 0.009 |
NS |
| 15 |
PUR9_ECO5S |
Bifunctional purine biosynthesis protein purH |
5.53/58 |
11 (14) |
31 |
NS |
+1.3 ± 0.2 |
+1.6 ± 0.06 |
| 16 |
DNAK_ECO24 |
Chaperone protein dnaK |
4.83/69 |
16 (28) |
28 |
NS |
+3.0 ± 0.1 |
+2.1 ± 0.2 |
| 17 |
AHPC_ECO57 |
Alkyl hydroperoxide reductase subunit C |
5.0/21 |
6 (11) |
43 |
NS |
+2.6 ± 0.3 |
+2.5 ± 0.4 |
| 18 | ALKH_ECO57 | KHG/KDPG aldolase | 5.57/22 | 7 (20) | 46 | NS | +1.8 ± 0.2 | +2.1 ± 0.3 |
a “-” indicates down-regulation and “+” indicates up-regulation. The numerical values indicate the relative intensities of protein spots of interest in comparison with the corresponding control proteins, and they are presented as mean ± standard deviations of at least 2 independent proteomic gels. “NS” indicates the intensity changes are statistically non-significant in comparison to the corresponding control proteins.