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. 2013 May 23;11:23. doi: 10.1186/1477-5956-11-23

Table 1.

Identification of differentially expressed proteins of E coli DH5α treated with PySSPy, PyRSPy and 3A

No. ICBI accession No. Protein identified Calculated pI/MW (kDa) Matched (Measured) peptide Sequence coverage (%) Relative intensitiesa
PySSPy PyRSPy 3A
1
GLYA_EC024
Serine hydroxymethyl-transferase
6.03/94
9 (22)
29
-25 ± 04
-2.1 ± 0.2
-1.4 ± 0.1
2
IR3Z2_ECOUT
Glycerol kinase
5.97/60
15 (30)
33
-2.3±0.4
-1.6±0.05
-1.7±0.1
3
DHSB_ECQU
Succinate dehydrogenase iron-sulfur subunit
6.327
10 (21)
47
-2.4 ± 0.1
-3.5 ± 0.3
-3.4 ± 0.1
4
KDUD_ECOLI
2-dehydro-3-deoxy-D-gluconate 5-dehyrixenase
5.24/27
8 (18)
40
+1.7 ± 0.1
+2.0 ± 0.2
+1.9 ± 0.2
5
IvEH_ECO24
Malate dehydrogenase
5.61/32
11 (22)
49
+2.3 ± 0.2
+3.1 ± 0.05
+1.9 ± 0.06
6
CH6O1_ECOK1
60 kDa chaperonin 1
4.85157
10 (26)
33
+2.4 ± 0.1
+2.5 ± 0.2
+3.1 ± 0.4
7
MAO2_ECOLI
NADP-dependent malic enzyme
5,3494
11 (13)
14
-21 ± 0.5
-2.1±0.3
NS
8
DPS_EC024
DNA protection during starvation protein
5.72/19
9 (1)
58
-2.3 ± 0.1
-2.8 ± 0.6
NS
9
PCKA_EC024
Phosphoenolpyruvate carboxykinase
5.46/60
14 (27)
35
-24 ± 0.2
NS
NS
10
GCST_EC024
Aminomethyltransferase
5.36/40
9 (17)
35
-2.1 ± 0.3
NS
NS
11
SODF_ECO57
superoxide dismutase [Fe]
5.58/53
15 (16)
37
NS
+1.9 ± 0.009
NS
12
ATPB_EC024
ATP synthase subunit beta
4.9150
16 (50)
50
+3.0±0.2
NS
+2.6 ± 0.2
13
EFTIJ1_EC024
Elongation factor Tu 1
53143
17 (44)
53
+3.3 ± 0.2
NS
+3.1 ± 0.1
14
TNAA_EC024
Tryptophanase
5.88/53
15 (16)
37
NS
+1.9 ± 0.009
NS
15
PUR9_ECO5S
Bifunctional purine biosynthesis protein purH
5.53/58
11 (14)
31
NS
+1.3 ± 0.2
+1.6 ± 0.06
16
DNAK_ECO24
Chaperone protein dnaK
4.83/69
16 (28)
28
NS
+3.0 ± 0.1
+2.1 ± 0.2
17
AHPC_ECO57
Alkyl hydroperoxide reductase subunit C
5.0/21
6 (11)
43
NS
+2.6 ± 0.3
+2.5 ± 0.4
18 ALKH_ECO57 KHG/KDPG aldolase 5.57/22 7 (20) 46 NS +1.8 ± 0.2 +2.1 ± 0.3

a “-” indicates down-regulation and “+” indicates up-regulation. The numerical values indicate the relative intensities of protein spots of interest in comparison with the corresponding control proteins, and they are presented as mean ± standard deviations of at least 2 independent proteomic gels. “NS” indicates the intensity changes are statistically non-significant in comparison to the corresponding control proteins.