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. 2013 May 31;8(5):e65071. doi: 10.1371/journal.pone.0065071

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© 2013 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Amino acid sequence differences between MDM selected 1690 M and 1690 T were shown in the regions of reverse transcriptase (A-C), Gag-Pol trans-frame (D), protease (E), Gag p2 (F) and Gag p6 (G). MDM from 3 different donors (Donor 1, 2 and 3) were infected with 1690 M and 1690 T, respectively. Supernatants were harvested on Day 22 post-infection and subjected to RT-PCR and sequence analysis on Gag-Pol regions of HIV-1. (2/2) represents the same individual sequences were detected from two RT-PCR. Differential residuals were indicated by both “*” and position numbers aligned with HXB2 sequences.